90 page Veterinary GENETIC ENGINEERING Presentation CD

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Numerous illustrations and matrices.

Contains the following key public domain (not copyrighted) U.S. Government publication(s) on one CD-ROM in both Microsoft PowerPoint and Adobe Acrobat PDF file formats:

TITLE:

Genetically Engineered Rodents, 2003, 90 pages

SLIDE TOPICS, SUBTOPICS and CONTENTS:

Genetically Engineered Rodents Transgenic Mouse Mouse with stably integrated exogenous DNA in its germ line. Uses of Transgenic Mice Tissue specific and developmental stage specific gene regulation Reporter genes (green fluorescent protein, Lac Z, Luciferase) Gene function or phenotypic effect of transgene expression Oncogenes Immune system genes Viral genes Developmental regulatory genes Hormones Protein production in large animals Gene knock down Tissue Specific Expression Using GFP M. Hara et al, Am J Physiol Endocrinaol Metab 284: E177-E183, 2003 Tissue Specific Expression Using LacZ Tissue Specific Expression Using LacZ Methods to Produce Transgenic Mice Pronuclear microinjection Viral infection of preimplantation embryos Nuclear transplantation Considerations in Construct Design Regulatory sequences Medline TBASE http://tbase.jax.org Cre recombinase transgenic database http://www.mshri.on.ca/nagy/ Intramural Animal Models Database http://ccr.ncifcrf.gov/tech_initiatives/animalmodels/db/ cDNA versus genomic DNA Transgene detection Transgene size (BAC, PAC, YAC) Transgene number Gene Transfer into Mouse Embryos Superovulation Strain Survey of Ovulation Response to Exogenous Gonadotropins Title Factors Critical to Successful Transgenic Mouse Production Presence of vector sequences Physical state of the DNA DNA concentration DNA purity Isolation of theDNA Fragment for Microinjection Agarose Gel of Fractions fromSucrose Gradients Detection of the Transgene Transgenic Rats Problems Associated with theDerivation of Transgenic Rats Derivation of Transgenic MiceUsing Lentiviral Vectors Binary Transgenics Knockout Mouse Production Knockout Mouse Production Velocigene: Integrated Solution Example of Velocigene Technology: BACvecs (For KOs or Tgs) then Introduced into ES Cells: Automated Genotyping of ES Cells: Post-transcriptional Gene Silencing (PTGS) and RNA Interference (RNAi) Methods for Generating siRNAs Methods for Generating siRNAs Key Applications of siRNA Animal Models